March. 12th

Today I stained the Day 14 Bone Marrow MSC in both normal growth medium (DMEM+10%FBS) and Osteo.   I first stained with the ALP Stain and waited 45 minutes.  I observed pink dots in the Osteo cell culture but not in DMEM. ALP stain the Alkaline Phosphatase enzyme, which is released by the differentiating cell […]

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Feb. 28

Today, we discussed about our schedule for cell culture for the next month. Our goal is to compare Bone Marrow MSC in growth medium with differentiating Bone Marrow MSC. It usually takes 21 days for MSC to differentiate, so for us, we will check after 15 days to compare the differentiation at different time point. […]

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Feb.19th

Today we pass the HBMC cell from passage 4 to passage 5 using the protocol I developed last time. We first counted the cell and using the formula, there are around 1.49 * 10^5 cells in the current flask. So to obtain the designated 3000 cells per cm square in a new 10 cm^2 round […]

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Feb.05th

Today I conducted the passage of the Huvec cell and developed a protocol for cell passage. Step 1: preparation Remove the cell medium from the flask Add 8mL DPBS to wash the cell so that we could wash away the dead cell Remove and discard DPBS Add 7mL of Triple Express enzyme and put the […]

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